Expertises >> Ecotoxicology >> Ecotoxicology


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 Download the Ecotoxicology brochure in pdf format


You would like:

  • To work out the ecotoxic potential of a complex substance or matrix
  • To ascertain the bioavailability of pollutants and possible interactions with environmental components
  • To understand the hazardous nature of your waste

Eurofins Expertises Environnementales provides the elements you need to study the impact of pollutants and their derivatives on living organisms in different ecosystems.


The characterisation of the environmental matrices with physical-chemical analyses does not always make it possible to predict the synergistic effects between the various compounds sometimes present at very low concentrations. The purpose of ecotoxicology is to study and predict, by extrapolation, the behaviour and effects of short-term and long-term pollutants in ecosystems.


How do you measure the toxicity?

By implementing biological tests on living aquatic and terrestrial organisms belonging to various trophic levels using standardised or bespoke methods.

The toxic effects are measured in the laboratory by exposing indicator organisms to the sample compared with a control. We rely on the principle of causality between the dose and the response. The results of these tests make it possible to complete the physical-chemical analyses and evaluate the risk of degradation of an ecosystem as a preventive measure. These tests can also be conducted after leaching/percolation to know and predict the transfer of chemicals from complex matrices, sludge, soil, sediment and waste in the aqueous medium.


Application areas

  • Registration of new chemical substances (phytosanitary substances, pesticides, treatment products, detergents, antifouling substances, etc.)
  • Monitoring of sewage from urban water treatment plants and industrial effluents (STEP inputs and outputs)
  • Ecotoxicity of special waste and assessment of the ecotoxicological risk of polluted sites
  • Inspection of landfill leachates, incineration residues, WWTP sludge, dredged sediments, etc.


Regulatory aspects

The law on water establishes the use of the bioassay with the daphnies test to identify the "inhibiting materials", a parameter integrated in the calculation of the water agency fee.

In order to introduce new substances and chemicals to the market according to the requirements of the OECD and the European Union, a declaration file that includes the physical-chemical, toxicological and ecotoxicological data must be submitted for its MA (Marketing Authorisation) to be passed.


A global offer

Freshwater organisms

  • Acute toxicity
  • Microcrustaceans (Daphnies NF EN ISO 6341, etc.)
  • Fish (Brachydanio rerio NF EN ISO 7346)
  • Bacteria (Microtox NF EN ISO 11348-3 test, NF EN ISO 9509 nitrification, activated sludge respiration OECD 209, etc.)
  • Chronic toxicity
  • Algae (NF EN ISO 8692)
  • Microcrustaceans (Daphnia: NF T90-378; cerodaphnias: NF ISO 20665
  • Rotifers (Brachionus: NF ISO 20666


Marine organisms

Acute toxicity

  • Algae (Phaeodactylum tricornutum NF EN ISO 10253)
  • Marine copepod (Acartia tonsa or Tigriopus brevicornis ISO 14669)
  • Crustaceans (Artemia salina)
  • Benthic crustaceans (Amphipod Corophium sp. NF EN ISO 16712)
  • Fish - bass (Larvae of Dicentrarchus labrax OECD 203)
  • Fish - bass (Immature juveniles of Dicentrarchus labrax OSPARCOM)
  • Corals : Bleaching and polyp retraction (Seriatopora hystrix – Internal method)

Semi-chronic toxicity

  • Embryonic development of bivalves (Crassostrea gigas or Mytilus sp)


Benthic organisms

  • Chironomes NFT 90-339-1
  • Ostracodes (Heterocypris incongruens - NF ISO 14371)


Terrestrial organisms

  • Acute and chronic tests / Plants (NF EN ISO 11269-2) / Earthworms (NF EN ISO 11268-1, -2, NF ISO 17512-1)


Genotoxic effects

  • Ames test in a liquid medium (Fluctuation test)



Information of the development of the sample is obtained by the chemical and/or ecotoxic monitoring of the samples placed under different degradation conditions (OECD 301A, 301B, 301D, 302B). These studies are used, for example, to evaluate effluent treatments or pollution control activities, but also to analyse the degradation of pure substances.


Endocrine disruptors

  • Amphibians (Xenopus laevis - According to XP-T90-716-1): Effect on the thyroid axis
  • Fish (Oryzias latipes - According to XP-T90-716-2): Effect on the oestrogenic axis


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